[By Gokul Ramaswami and Robert Piskol. Gokul Ramaswami is a graduate student and Robert Piskol is a postdoctoral fellow in the Department of Genetics at Stanford University. Both study RNA editing with Jin Billy Li.]
Thank you to Genomes Unzipped for giving us the opportunity to write about our paper published in Nature Methods [1]. Our goal was to develop a method to identify RNA editing sites using matched DNA and RNA sequencing of the same sample. Looking at the problem initially, it seems straightforward enough to generate a list of variants using the RNA sequencing data and then filter out any variants that also appear in the DNA sequencing. In reality, one must pay close attention to the technical details in order to discern true RNA editing sites from false positives. In this post, we will highlight a couple of key strategies we employed to accurately identify editing sites.
Read the rest of this entry | Read comments